Our work on nanopore sensing of ITP-focused analytes is out now in Nanoscale
Microfluidic device for coupling isotachophoretic sample focusing with nanopore single-molecule sensing
Nanopore sensing remains challenging at low target concentrations, where the detection rate is limited by slow diffusion of analytes into the sensing volume. In this work, we show that an electrokinetic preconcentration method called isotachophoresis (ITP) can be used to deliver a highly concentrated zone of analytes to a solid-state nanopore. The key to ITP-nanopore sensing lies in the ability to electrically decouple the two modes to avoid damaging the fragile dielectric membrane. By choosing electrolyte conditions that enable both efficient focusing and sensing, and by implementing electrical switching in a custom microfluidic device, we show that ITP focusing can enhance the event rate by over 300 times compared to free diffusion.
Welcoming Neeraj at Hunta bar
Celebrating our new student Neeraj joining the lab at the “Hunta pub” Technion
Karawan Halabi and Noam Freundlich Welcome to the Lab!
Karawan and Noam are new MSc students in our lab. Both completed their degree in Biomedical Engineering this year. Karawan and Noam will be joining our protein identification team.
Read Amit's N&V article in Nature Nanotechnology
Scanning Nanopore Microscopy - A new tool for cell signalling research
The combination of a nanopore and an atomic force microscope allows stochastic sensing of secreted molecules and the activity of ion channels in arbitrary locations both inside and outside of a cell. In this News & Views article I highlight recent paper by Aramesh and colleagues in Nature Nanotechnology.
Azrieli Fellowship awarded to Shilo Ohayon
39 new Azrieli Fellows selected for 2018/19 The Azrieli Fellows Program has completed its annual selection process, granting 25 new Fellowships for graduate students in the fields of Sciences, Social sciences, Education, Humanities and Architecture & Urban Planning, 6 international postdoctoral Fellowships for research in varied fields and 8 Faculty Fellowships for returning scholars.
Since the Program’s inception in 2007, more than 250 of the best and brightest researchers at universities in Israel have been supported.
Simulation of single-protein nanopore sensing shows feasibility for whole-proteome identification
Our simulations study show feasibility of whole proteome nanopore sensing. Shilo, Arik and Maisa’s paper final version is published in the Open Access journal PloS Computational Biology Congratulations!