Maisa and Eran are new MSc students in our lab. Maisa has recently completed a joint BSc degree in Physics and Biomedical Engineering, and Eran completed his degree in Biomedical Engineering. Maisa and Eran will be joining our Nanopore team.
On October 26, 2018, Prof. Meller delivered the Joan van der Waals colloquium in the Department of Physics, Leiden University, the Netherlands. The broadly accessed colloquium was entitled “Nanopore bio-sensing: past, present and future” and covered over 20 years of nanopore research towards DNA sequencing as well as recent developments leading to
In a recent publication (ACS Nano, November 2018, DOI: 10.1021/acsnano.8b07055) we showed that thin titanium dioxide membranes produce negligible photoluminescence background as compared to silicon nitride membranes of same thickness. This discovery permits electrooptical sensing in nanopores with much improved signal-to-background ratio, allowing us to use it for sensing and discrimination among labelled DNA strands as well as polypeptides for the first time.
Proteins are the structural elements and machinery of cells responsible for a functioning biological architecture and homeostasis. Advances in nanotechnology are catalyzing key breakthroughs in many areas, including the analysis and study of proteins at the single-molecule level. Nanopore sensing is at the forefront of this revolution. This tutorial review, published on October 17, 2018, provides readers a guidebook and reference for detecting and characterizing proteins at the single-molecule level using nanopores. Specifically, the review describes the key materials, nanoscale features, and design requirements of nanopores. It also discusses general design requirements as well as details on the analysis of protein translocation. Finally, the article provides the background necessary to understand current research trends and to encourage the identification of new biomedical applications for protein sensing using nanopores.